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Cell Biochemistry Martinsried |
1. Introduction:
The overtraining syndrome as a consequence of prolonged physical exercise
leads to reversible episodes of substantially lowered physical strength in
combination with an increased susceptibility to opportunistic infections
which may lead to life threatening disease. There are no early indicators
of a beginning overtraining syndrome.
2. Goal:
Early identification of endurance athletes in danger of overtraining
syndrome by flow cytometric immunophenotyping of peripheral blood
lymphocytes.
3. CLASSIF1 Data Pattern Classification:
A database was calculated from the CD45RO/CD4, CD45RO/CD8,
CD3/HLA-DR, CD3/CD16, CD19/blank two colour
assays in conjunction with simulatneous forward(FSC) and
sideward (SSC) light scatter analysis for a total of 72
normal/overtrained competition cyclists.
Since no significant changes of the usual % frequency values of the various lymphocyte populations were observed, an exhaustive analysis of the flow cytometric list mode files was performed with 5x34=170 database columns instead of only 5x4=20 database columns on % cell frequency analysis alone.
Standardized and automated data classification with the CLASSIF1 multiparameter data classification program permitted the >95% single case recognition of overtrained cyclists. The development of the overtraining syndrome was paralleled by a reversible increase of CD45RO antigen expression on the lymphocyte surface. Monitoring this increase with time may be a means to avoid the occurrence of overtraining syndromes in endurance athletes.
4. Conclusion:
The determination of lymphocyte CD45RO antigen expression
(antigen surface density) provides a
>95%
correct identification of
an imminent overtraining syndrome. The relative frequency
of the various lymphocyte populations, in contrast, is
non informative.
© 2024 G.Valet |