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6th ESACP Congress, Heidelberg, April 7-11, 1999 |
A063
Although considerable knowledge exists on clinical-pathologic, cytometric and
genetic characteristics of larynx-pharynx carcinomas (LPC), still little is
known about the relationship with tumor progression. DNA was extracted from
frozen tissue of 16 LPC, and analysed by comparative genetic hybridization
(CGH). Results were related to DNA ploidy, tumor localization and pTNM data.
A high number of gains and losses was found: on average 8.3 and 5.4 per case,
respectively. The highest number of gains were observed at 3q (13 cases),
8q (10 cases), 7q and 1q (7 cases), 17q and 2q (6 cases). Recurrent losses
occurred at 3p (8 cases), 4q, 18q and 21q (5 cases). High level amplifications
were found at 3q26-27 (5 cases), 11q13 (3 cases), 11q22 and 18q11 (2 cases),
7p22, 8q24, 13q34 and 22q11 (1 case). We found no differences in the average
number or location of chromosomal changes in relation to DNA ploidy, tumor
localization and pTNM stage. However, nearly all high level amplifications
were displayed in larynx cases.
We can conclude that chromosome 3 (3q gain and 3p loss) could have a high
importance in the LPC progression. More cases are being investigated and the
genetic alterations will be studied in relation to clinical follow-up data.
CHROMOSOMAL GAINS AND LOSSES IN LARYNX AND PHARYNX CARCINOMAS BY COMPARATIVE
GENOMIC HYBRIDIZATION (CGH)
Guervós MA 1, Hermsen M 2, Álvarez-Marcos C 3, Salas A 1, Sampedro A 1,
Van Diest PJ 2
1) Cytometry Service, University of Oviedo, Spain; 2) Dept. Pathology, Free
University Hospital, Amsterdam, Netherlands; 3) Dept. Otolaryngology,
Valle Del Nalón Hospital, Asturias, Spain