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6th ESACP Congress, Heidelberg, April 7-11, 1999 |
A043
RS cells observed in Hodgkines disease are large, tetra- or
polyploid cells with bi- or multilobed nuclei and cell surface antigens
claimed to be positive for CD 15, 30 and 70. They are considered to be
of disputed lineage and ambivalent clonality. RS-like cells have also been
reported in solid tumours. We shall report here on features of RS-like cells
in mitotic and interphase stages observed in the bone marrow of myelodysplastic
syndromes (MDS) and severe aplastic anaemia (SAA). Higher magnifications
(63x or 100x oil immersion) revealed binucleate cells with generally
tetraploid nuclei with bi- or multilobed morphology, demonstrating prominent
bilateral or mirror image symmetry as shown by the distribution of chromosome
specific FISH signals. In one patient with a high frequency of RS-like cells
a metaphase showed a mirror image symmetry punctuated by the sex chromosome
FISH signals. Similar symmetric chromosome distribution have been observed
in diploid bilobed nuclei of granulocytes (Chaudhuri, Reith. Analytical
and Quantitative Cytology and Histology, 1997, 19: 30-36). The reported
features of these RS-like cells in mitotic and interphase stages may have
implications on a better understanding of their cytopathology, cytogenetics
and clonality.
BILATERAL OR MIRROR IMAGE SYMMETRY OF CHROMOSOME DISTRIBUTION IN
BINUCLEATE REED-STERNBERG (RS)-LIKE CELLS BY FISH SIGNALS
Chauduri JP, Führer M, Reith A, Walther JU
Kinderpoliklinik, LMU-München, Germany, The Norwegian Radiumhospital
and Cancer Institute, Oslo, Norway