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6th ESACP Congress, Heidelberg, April 7-11, 1999 |
A150
BACKGROUND The histopathological differentiation of well-differentiated
carcinomas and benign atypic adenomas of sweat gland origin may be
difficult, even if immunohistochemical methods are used in addition. We could
demonstrate in a previous study, that DNA image cytometry (ICM-DNA) can be
helpful in assessing the dignity of recurrent clear cell hidradenoma. In
this study, a larger series of sweat gland tumors, clear-cut malignant or
benign by histopathological criteria, was examined by ICM-DNA. MATERIALS
AND METHODS 16 sweat gland carcinomas and 41 benign sweat gland tumors were
examined. DNA image cytometry ( ICM-DNA ) was conducted according to the
actual recommendations of the ESACP task force on standardization of ICM-DNA
on enzymatic cell separation specimens of paraffin embedded material with
the Autocyte Quic-DNA workstation using mesenchymal cells as internal
reference. DNA-aneuploidy was detected by the stemline interpretation
according to Böcking and/or 3 or more 5c-exceeding events. A classifier for
sweat gland tumors could be constructed out of 37 DNA-euploid stemlines of
the benign tumors by use of the EUROQUANT server, Dresden (Haroske et al. 1998).
RESULTS DNA-aneuploidy was detected in 56,25% (9/16) of the sweat gland
carcinomas, but in none of the 41 adenomas. CONCLUSIONS The detection of
DNA-aneuploidy in sweat gland tumors using ICM-DNA seems to be a clear and
specific indicator of prospective malignancy. However, regarding a
sensitivity of about 56%, malignancy cannot be excluded if DNA-aneuploidy is
not detected in a sweat gland tumor.
DNA IMAGE CYTOMETRY (ICM-DNA) IN HUMAN SWEAT GLAND TUMORS
Vogelbruch M *, Böcking A **, Kapp A *, Kiehl P ***
* Department of Dermatology and Allergology, Hannover Medical University,
Germany
** Institute of Cytopathology, University of Düsseldorf,
*** Dermatohistopathology and Electron Microscopy Unit, Hannover, Germany.