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6th ESACP Congress, Heidelberg, April 7-11, 1999 |
A068
In the threedimensional analysis of the nucleus of the cell, a high
resolution in all three directions of space is of major importance. Images
optained with a standard confocal microscope have the drawback of a
comparatively small resolution along the optical axis. By rotating the cell
under the microscope it is possible to obtain confocal images where the axial
direction points into different directions inside the cell. A new technique
for computation of a high-resolution three dimensional image from a few
datastacks obtained with this axialtomographic method will be presented.
Such reconstructed images are shown to have a resolution in all three
directions comparable to the high lateral resolution of deconvolved confocal
images. Modell-data from a chromosomal territory were simulated using
standard confocal microscopy with deconvolution and this axialtomographic
technique with reconstruction.
RECONSTRUCTION OF AXIALTOMOGRAPHIC HIGH RESOLUTION DATA FROM A CONFOCAL
MICROSCOPE
Heintzmann R, Kreth G, Cremer C
Applied Optics and Information Processing, Institute of Applied Physics,
University of Heidelberg, Germany