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6th ESACP Congress, Heidelberg, April 7-11, 1999 |
A097
Intracellular glutathione (GSH), gamma-glutamyltransferase (gamma-GT)
and glutathione-S-transferase (GST) antioxidative enzymes involved in
GSH metabolism, immunophenotype profile and apoptotic cell death were
investigated in peripheral blood mononuclear cell (PBMC) isolated from
patients with insulin-dependent type I (IDDM) and non insulin-dependent
type II (NIDDM) diabetes mellitus. There was no correlation between any of
the measured parameters and the quality of diabetic control (HbA1c level).
The absolute number of lymphocytes was increased whilst the percentage of
CD3, CD4 and CD 8 T-lymphocytes was significantly reduced in both IDDM and
NIDDM patients. Furthermore the absolute number and percentage of
B-lymphocytes was found higher in IDDM than in NIDDM patients and healthy
donors. PBMC from diabetic patients exhibited a slight yet significant
reduced level of GSH and a significant dysregulation of the activity for both gamma-GT
and GST enzymes. This accounted for a minor compromission of the oxidative status
which appreared to be linked to the low plasma membrane permeability to
propidium iodide and indicated that the rate of apoptotic cell death was
reduced in PBMC from diabetic patients.
APOPTOTIC CELL DEATH AND OXIDATIVE STATUS IN LYMPHOCYTES FROM
TYPE I / TYPE II DIABETIC PATIENTS
Losa GA, Graber R
Laboratorio di Patologia Cellulare, Istituto di Patologia,
Locarno, Switzerland