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6th ESACP Congress, Heidelberg, April 7-11, 1999 |
A033
Investigation of glycogen function in hepatocytes of different liver lobule
zones is particularly important in understanding glycogen metabolism in human
and animals in norm and pathology. Glycogen content was determined in
hepatocytes of different lobule zones of the normal human liver and the
liver of patients with liver cirrhosis of viral or alcohol etiology.
Quantitative analysis of glycogen content in hepatocytes of portal and
central lobule zones was conducted on slices of human liver (the material
of series live punctional biopsies) stained using a quantitative variant
of PAS-reaction (Kudryavtseva et al., 1970; 1974). The measurements were
done by image analyzer "Videotest". The results showed clear differences of
glycogen contents in different lobule zones in normal liver and in liver
during viral and alcohol cirrhosis. Glycogen contents in hepatocytes of
portal lobule zone were significantly higher than in the central lobule
zone in patients with viral cirrhosis. Opposite data were obtained in
patients with alcohol cirrhosis. Significantly higher glycogen contents were
found in hepatocytes of the central lobule zone. Thus, the pattern of the
glycogen content in hepatocytes of different lobule zones can be used as an
indicator of etiology of liver cirrhosis.
QUANTITATIVE ANALYSIS OF METABOLIC HETEROGENEITY OF GLYCOGEN IN HEPATOCYTES
IN PATIENTS WITH LIVER CIRRHOSIS.
Bezborodkina NN, Kudratseva MV, Kudratsev BN
Institute of Cytology, Russian Academy of Sciences,
St.Petersburg, Russia