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6th ESACP Congress, Heidelberg, April 7-11, 1999 |
A161
The apple brandy "calvados" and other spirits are strongly related to
the risk of oesophageal squamous cell carcinoma when consumed with hot
beverages and/or tobacco. In order to study the relationship between
exposure to alcoholic beverages, tobacco, practice of drinking spirits and
incidence of precancerous lesions in oesophagitis area, a retrospective
study was carried out on oesophageal mucosa biopsies collected in a region
with high incidence for oesophageal cancer, namely Normandy. 22 biopsies from
healthy volunteers were analyzed and compared to non neoplastic mucosa
collected on 12 surgical specimen of cancer resection at distance of the
tumour. Epidemiological data concerning smoking, alcohol consumption and
alimentary habits were available for all individuals. Formalin fixed and
paraffin embedded histological sections were immunostained for the Ki67
proliferation marker and the p53 tumor suppressor protein. A quantitative
study of the distribution of Ki67 positive nuclei was performed in the tree
layers of the epithelium using a stereological approach. Chronic oesophagitis
was observed in 100 % of the patients and 68 % of the volunteers. An
increased proliferative rate related to the severity of oesophagitis was
observed in all specimen presenting an oesophagitis, especially in the
suprabasal layers and along the elongated intraepithelial stoma papillae.
p53 immunostaining was also observed in oesophagitis areas.In an attempt
to correlate proliferative activity, degree of oesophagitis and
epidemiological data, two quantitative informations were obtained from the
same histological sections of mucosa: Ki67 score expressed as the numerical
density of immunostained nuclei and a B/L ratio which corresponds to the
length of basal cell layer per unit length of exposed superficial epithelial
layer. A significant correlation (p=0.04) was obtained in healthy volunteers
between B/L ratio and consumption of hot alcoholic beverages, in particular
calvados. These preliminary results underline that prevalence of chronic
oesophagitis in high risk population could be related to drinking spirits
with hot beverages.
INFLUENCE OF CONSUMPTION OF HOT ALCOHOLIC BEVERAGES ON EXPRESSION OF KI67
PROLIFERATION MARKER, P53 PROTEIN AND CHRONIC OESOPHAGITIS OCCURRENCE
IN HUMAN OESOPHAGEAL MUCOSA
Lechevrel M 1, Launoy G 1, Marnay J 2, Sichel F 1, Gauduchon P 1,
Mandard AM 2
1 Centre F.Baclesse and CHRU, GRECAN, Caen,
2 Laboratoire d'Anatomiue Pathologique, Centre F. Baclesse, Caen, France