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6th ESACP Congress, Heidelberg, April 7-11, 1999 |
A051
Malignancy Associated Changes represent nuclear texture alterations found at
the vicinity of many cancers, which can only be observed by high resolution
image analysis. Recently, we demonstrated MAC feature expression at the
vicinity of laryngeal cancer. The aim of this study was to confirm this
finding at a larger sample set and to correlate this feature to the
inflammatory response of the stroma. This study comprises histological
sections of benign laryngeal epithelium with (n=20) and without lymphocytic
infiltrate (n=20) as well as benign appearing epithelium at the vicinity of
laryngeal cancer with (n=21) and without lymphocytic stromal infiltrate
(n=21). Typically 150 Feulgen Thionin stained cell nuclei of the epithelium
of each specimen were scanned by a Cyto Savant image analyzer (Oncometrics,
VancouverBC). The most important result was that nuclear texture features
indicating nearby cancer were only detected in the presence of lymphocytic
stromal infiltrate. Cell by cell classification accuracy was 69%. The benign
control samples indicated that this phenomenon could not be explained by non
specific inflammatory conditions or healing effects of the epithelium. From
these data we conclude that lymphocytes play a key role in MAC feature
expression by carrying a specific signal to benign epithelial cells at the
vicinity of cancer.
MALIGNANCY ASSOCIATED CHANGES: A NEW DIAGNOSTIC TOOL IN EARLY LARYNGEAL
CANCER DETECTION?
Dreyer Th 1, Garner D 3, Doudkine A 3, Knoblauch I 1,
Popella Ch 2
1) Institute of Pathology and 2) Department of Otorhinolaryngology
of the Justus Liebig University of Giessen, Germany; 3) BC Cancer Research
Centre, Vancouver, Canada