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6th ESACP Congress, Heidelberg, April 7-11, 1999 |
A160
A series of 102 normal mucosas, 102 oral non-dysplastic leukoplakias, 88 oral
dysplastic lesions and 102 squamous cell carcinomas, were evaluated in
paraffin-embedded sections using E29 Epithelial Membrane Antigen, EMA,
monoclonal antibody. Quantitative analysis of immunoreactivity (EMA positive
surfaces and percentage of immunopositive cells) was assessed. EMA
immunostaining had high ability to establish the difference between benign
and malignant cells of squamous epithelia. Its distribution was detected with
typical patterns for each type of mucosa. In normal, non-dysplastic lesion,
dysplasia and carcinoma, the quantitative immunohistoanalysis found 1.8%±1.2;
4.6%±2.2; 21.6%±15.2 and 35.5%±22.7 of EMA-positive surfaces, respectively;
and the cases of more than 20% EMA positive cells were 0%; 0%; 37.5% and
98%, respectively. EMA affinity, both in premaligant lesions and in squamous
cell carcinomas, was correlated with significant changes of the proliferating
cellular nuclear parameters. The percentage of EMA positive cells showed a
significant correlation with mitotic index, diploid index (p< 0.01),
hyperploid degree (p< 0.01) and AgNORs size (p< 0.01) in carcinomas.
The EMA expression within squamous epithelial cells has to be investigated
by quantitative immunohistochemical methods and used as a diagnostic
indicator.
EPITHELIAL MEMBRANE ANTIGEN QUANTITATIVE IMMUNOCYTOCHEMICAL ANALYSIS IN ORAL
LESIONS AND ORAL SQUAMOUS CELL CARCINOMAS
Fernandez Alvarez BE 1, Lopez-Muñiz 1, Anitua MJ 1, Triviño A 1,
Garcia-Pola JMJ 2
1 Dept. Morphology and Cellular Biology,
2 Dept. Oral Surgery, School of Dentistry, Faculty of Medicine,
University of Oviedo, Spain