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6th ESACP Congress, Heidelberg, April 7-11, 1999 |
A089
By cytofluorometric method, a study was made of the total glycogen and
its two fractions in liver parenchymal cells both in the donors and
in patients with cirrhosis of different etiology. The examination was
performed on preparations-smears of isolated hepatocytes, obtained of the
live punctional liver biopsies. The quantitative analysis has been shown an
increase in the total glycogen content in hepatocytes of patients with
cirrhosis by 3 times compared to the norm, and this increase is independent
on the etiology of liver cirrhosis. To study the mechanism of the discovered
glycogenosis, the activity of key enzymes of glycogenolyses was determined.
It was shown that glucose-6-phosphatase and glycogen phosphorylase activity
in the liver with cirrhosis was lower than in the norm. The most considerable
changes were shown in hepatocytes of patients with liver cirrhosis in
fractional glycogen composition and, even more significant, in the content
of a hard soluble fraction. The hard soluble fraction portion was higher in
hepatocytes of the patients with liver cirrhosis of alcohol etiology. The
quantitative analysis of glycogen fraction contents in liver cells may be
the best marker in the differential diagnosis of symptomless elapsing liver
cirrhosis.
CYTOFLUOROMETRIC ANALYSIS OF GLYCOGEN IN HEPATOCYTES IN PATIENTS
WITH LIVER CIRRHOSIS
Kudryavtseva MV, Bezborodkina NN, Kudryavtsev BN
Institut of Cytology, Russian Academy of Sciences, St.Petersburg, Russia