![]() |
6th ESACP Congress, Heidelberg, April 7-11, 1999 |
A024
Laryngeal carcinomas are often preceded by clinically and histopathologically
recognizable lesions which do not fulfill the criteria for malignancy. We
have used the In Situ Hybridization (ISH) technique to study the presence
of numerical aberrations of the chromosomes 1 and 7 in thin sections from
premalignant laryngeal lesions. Based on these ISH results specific areas
were isolated by laser-facilitated microdissection followed by a fluorescent
microsatellite analysis for the detection of loss of heterozygosity (LOH) of
the chromosome regions 9p21 and 17p13. Numerical aberrations were detected
in only 1 of 11 hyperplastic lesions without dysplasia, whereas most lesions
with dysplasia (23 of 26) contained aberrations for these chromosomes. LOH
for 9p21 and/or 17p13 was not detected in 5 hyperplastic lesions, whereas
10 of 12 lesions with dysplasia showed LOH for one or both loci. These
results show that both numerical chromosomes aberrations and loss of
specific chromosome regions are detected in an early phase of laryngeal
carcinogenesis. By using this multi-technique approach, we aim to obtain a
detailed picture of the amount of genetic changes present in these lesions.
In the near future, these findings may be of importance to identify
premalignant laryngeal lesions at risk for malignant transformation.
LOH AND ANEUPLOIDIZATION IN PREMALIGNANT LARYNGEAL LESIONS
Veltman J 1, Aubele M 2, Bosch F 3, Bot F 4, Ramaekers F 5, Manni J 1,
Hopman A 5
1) Dept.Otorhinolaryngology, University Hospital Maastricht, Netherlands,
2) GSF-National Center for Environment and Health & Institute of Pathology,
TU-Munich, Germany,
3) Molecular Biology Laboratory, ENT-University Hospital, Heidelberg, Germany,
4) Dept.Pathology, University Hospital Maastricht, Netherlands,
5) Dept.Molecular Cell Biology and Genetics, University of Maastricht,
Netherlands