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6th ESACP Congress, Heidelberg, April 7-11, 1999 |
A094
Objective: To compare DNA ploidy in fresh/frozen samples of endometrial
carcinoma assessed by flow cytometry (FCM) and image cytometry (ICM).
Study design: In samples from 154 tumours the DNA ploidy (diploid,
nondiploid) content was determined by quantitative analysis of nuclear DNA
content. Tissue suspension from the frozen samples were used for FCM and
imprint preparations were used for ICM and to ensure the presence of
preserved tumour cells. The ploidy in the FCM and ICM histograms was based
on number of stemlines. Furthermore the ICM histograms were also interpreted
according to the Auer classification used for breast carcinoma.
Results: 117 samples were FCM diploid, of these ICM showed diploidy in 89
(76%) and nondiploid in 28 (24%). 37 samples were FCM nondiploid, all 37
were also nondiploidy by ICM (100%). The comparision between the FCM data
and the Auer classification is tabulated
DNA PLOIDY ANALYSIS OF ENDOMETRIAL CARCINOMA. COMPARISON OF FLOW CYTOMETRY
AND IMAGE CYTOMETRY ON FRESH/FROZEN TISSUE.
Lindén Th *, Akerman M *, Baldetorp B +, Ingvaldson L *,
Fernö M +
* Department of Pathology/Cytology, University Hospital, Lund,
+ Department of Oncology, University Hospital, Lund, Sweden.
FCM Diploid |
FCM Nondiploid |
|
Auer 1 | 49 | 0 |
Auer 2 | 19 | 0 |
Auer 3 | 41 | 4 |
Auer 4 | 9 | 33 |
Total | 117 | 37 |
Conclusion: There was a good agreement between the FCM an ICM nondiploid cases. However, 24% of FCM diploid tumours were nondiploid in ICM. In assessment of DNA ploidy status in endometrial carcinoma and ICM should be combined to avoid false diploid FCM histograms. Evaluation according to the Auer classification was not more informative than the determination of the number of stemlines.