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6th ESACP Congress, Heidelberg, April 7-11, 1999 |
A098
There are many different modes of microscopy. All of these modes deliver
light in a controlled fashion to the object to be examined and collect as
much of the light containing the desired information about the object as
possible. The system being presented replaces the simple irises or annuli of
a conventional microscope with digital light processing units (digital
micromirror devices, or DMDs, made by Texas Instruments) to produce a digital
microscope. The DMDs are placed in the optical path at positions
corresponding to conjugate image planes of the sample and the aperture
diaphragm of a conventional microscope. This allows for more precise and
flexible control over the spatial location, amount, and angles of the
illumination light and the light to be collected. This digital microscope will
introduce improvements to existing modes of microscopy, specifically in the
field of quantitative microscopy. In various configurations, one can
perform brightfield, darkfield, confocal, and fluorescence microscopy. In
addition, new modes of microscopy will be possible, such as reconstruction
microscopy. Using the intensity modulation feature of the DMDs, the system
can also correct for inhomogeneous illumination sources to achieve uniform
distributions. Alternatively, one can project images or information into the
microscope field of view for specific applications.
AN UPDATE ON THE USE OF DIGITAL MICROMIRROR DEVICES IN QUANTITATIVE
MICROSCOPY
MacAulay C, Dlugan A
Cancer Imaging, BC Cancer Agency, Vancouver, Canada