6th ESACP Congress, Heidelberg, April 7-11, 1999

A081
PROGNOSTIC SIGNIFICANCE AND COMPARISON BETWEEN IMAGE CYTOMETRY AND FLOW CYTOMETRY IN DNA PLOIDY DISTRIBUTION IN EPITHELIAL OVARIAN CANCER STAGE 1
Kildal W 1, Kristensen GB 2, Abeler VM 1, Pettersen EO 1 Danielsen HE 1

1) Depts.Pathology and 2) Gynecologic Oncology, The Norwegian Radium Hospital, Montebello Oslo, Norway

In patients with ovarian cancer, parameters such as FIGO staging, histopathologic type and grading are of important prognostic value. The last decade DNA ploidy distribution has also become an important prognostic tool. In the present study we have assessed about 250 cases of ovarian cancer Stage I, taken from patients treated at the Norwegian Radium hospital from 1982 to 1989, with both flow cytometry (FCM) and image cytometry (ICM). All tumors were staged according to FIGO and histologically verified by light microscopy of hematoxylin-eosin stained sections. Histological samples were sliced into 50 micrometers (FCM) and 100 micrometers (ICM) sections, deparaffinized, and stained with ethidiumbromide and feulgen, respectively. Preliminary data suggests approximately 80 % correlation between ploidy and prognosis, and the correlation between FCM and ICM is about 85 %. These data will be presented in detail on the ESACP congress.