6TH ESACP CONGRESS

HEIDELBERG

7-11 APRIL 1999

Apoptosis Tutorial

7th April, p.m.

Tutors: Michael Ormerod, Reigate, UK

Zbigniew Darzynkiewicz, New York, USA

  1. Michael Ormerod
    1. Role of apoptosis
    2. Properties of apoptotic cells
    3. The apoptotic cascade
    4. Using flow cytometry to quantify events in the apoptotic cascade
  2. Zbigniew Darzynkiewicz
    1. Methods for recording changes in DNA and chromatin in apoptotic cells
    2. Simultaneous measurement of apoptosis and proliferation
    3. Clinical applications
  3. Zbigniew Darzynkiewicz
    1. Using Laser Scanning Cytometry in the study of apoptosis
  4. Examples of flow cytometric analysis of apoptotic cells (Time permitting)

This session will use listed data to run through the analysis in real time (limited to a small tutor group).

Some key references

  1. Bedner, E., Li, X., Gorczyca, W., Melamed, M.R. and Darzynkiewicz, Z. (1999) Analysis of apoptosis by laser scanning cytometry. Cytometry 35: 181-195.
  2. Darzynkiewicz, Z., Bruno, S., Del Bino, G., Gorczyca, W., Hotz, M.A., Lassota, P. and Traganos, F. (1992) Features of apoptotic cells measured by flow cytometry. Cytometry 13: 795-808.
  3. Darzynkiewicz, Z., Juan, G., Li, X., Gorczyca, W., Murakami, T. and Traganos, F. (1997) Cytometry in cell necrobiology: analysis of apoptosis and accidental cell death (necrosis). Cytometry 27: 1-20.
  4. Darnzynkiewicz, Z., Li, X. and Gong, J. (1994). Assays of cell viability: discrimination of cells dying by apoptosis. In Flow Cytometry. Darzynkiewicz, Z. Robinson, J.P. and Crissman, H.A. (eds.) Methods in Cell Biology, 41, Academic Press, Inc., San Diego. pp15-38.
  5. Fraker, P.J., King, L.E., Lill-Elghanian, D. and Telford, W.G. (1995). Quantification of apoptotic events in pure and heterogeneous populations of cells using flow cytometry. In Cell Death. Schwartz, L.M. and Osborne, B.A. (eds.) (1995). Methods in Cell Biology, 46, Academic Press, San Diego. pp 57-76.
  6. Ormerod, M.G. (1998) The study of apoptotic cells by flow cytometry. Leukemia 12, 1013-1025.
  7. Sherwood, S.W. and Schimke, R.T. (1995). Cell cycle analysis of apoptosis using flow cytometry. In Cell Death. Schwartz, L.M. and Osborne, B.A. (eds.) (1995). Methods in Cell Biology, 46, Academic Press, San Diego. pp 77-98.