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6th ESACP Congress, Heidelberg, April 7-11, 1999 |
A153
DNA image cytometry (ICM) proved to be a valuable tool in follow up of cancer
patients. Yet, the lack of generally accepted numerical classification of
DNA-histograms limits its value. The aim of the presented study was to test
the discriminative value of DNA parameters suggested by the European
Consensus report. On smears of 100 pleural effusions from cancer patients a
panel of chromosomes was tested for numerical aberrations. Direct
fluorescence in situ hybridisation was done for chromosome 7, 8, 11, 12, 17
and 18 on all effusions. Image cytometrically obtained DNA-histograms were
classified by the parameters DNA-Index (DI), 2c deviation index (2cDI)
according to Böcking, and 5cex-rate. Values found in pleural effusions
lacking any numerical chromosomal aberrations were compared to those with
numerical chromosomal irregularities. Independently achieved data were
correlated. In the group lacking any numerical chromosomal aberrations the
parameters DI, 2cDI and 5cex-rate ranged from 0.84-1.32 (mean 0.99),
0.03 - 0.68 (mean 0.18) and 0.00-0.87 (mean 0.04), respectively. The group
of effusions with low and marked numerical aberrations showed DI, 2cDI
values and 5cex-rates between 0.91 and 3.03 (mean 1.33), 0.04 and 23.53
(mean 1.95), and 0.0 and 84.47 (mean 5.14). Although the mean values differ
significantly, results demonstrate that the 2cDI and 5cex-rate cannot be
used as single descriptors in ICM. Their combinatory use with e.g. the
DNA-index appears to be of higher value.
NUMERICAL CHROMOSOMAL ABERRATIONS EVALUATED BY FISH - A BASIS FOR
STANDARDIZATION IN IMAGE CYTOMETRTY OF PLEURAL FLUIDS?
Wiener HG, Remkes GW, Kaufmann H, Fiegl M, Breitnecker G
Departments of Pathology and Oncology, Universities Vienna and Innsbruck,
Austria