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6th ESACP Congress, Heidelberg, April 7-11, 1999 |
A060
The ESACP task force consensus document in DNA image cytometry originally
ratified in Grenoble-France on May 17th 1994 and updated in
Oslo-Norway on May 27th 1997, is actually in press in the ACP journal. The
consensus is open for forum discussion through the ESACP server for further
development. The QC events will be introduced sequentially and illustrated
by data obtained from partners in the PRESS project and users of EUROQUANT
server. A simulation of various situations will be presented. Briefly, the
QC procedure includes six tests: 1) QC for ICM-DNA protocol, 2) QC for
preparation stability, 3) QC for corrective factor, 4) QC for diagnostic DNA
interpretation, 5) QC for IOD measurements and 6) QC for ICM instrumentation.
At the starting point, a new user, is invited to run the QC for ICM-DNA
protocol. Depending on the positive or negative issue of this first test,
the user will be invited either to run clinical cases or to check his
protocol. Checking his protocol needs two successive steps: QC for IOD
measurements and QC for ICM instrumentation. Those ones should help the
user to solve the methodological problems encountered when running his
laboratory protocol: fixation, staining, image analysis procedure. As soon
as the QC for ICM-DNA protocol leads to a positive issue, the user is
invited to run clinical samples associated to their respective external and
internal references appropriate for evaluation of both stability of protocol
(QC for preparation stability) and data calibration (QC for corrective
factor). Finally QC for diagnostic DNA interpretation is required.
QUALITY CONTROL AND QUALITY ASSURANCE PROTOCOLS FOR DNA IMAGE CYTOMETRY
Giroud F 1, Haroske G 2
1) Equipe RFMQ/TIMC, Université Joseph Fourier, Grenoble, France,
2) Institute of Pathology, Dresden University of Technology, Dresden, Germany