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6th ESACP Congress, Heidelberg, April 7-11, 1999 |
A142
Recent development of in vivo microscopy techniques, including green
fluorescent proteins (GFP), has allowed the visualization of a wide range
of dynamic processes in living cells. For quantitative and visual
interpretation of such processes, new concepts for time-resolved image
analysis and continuous time-space visualization are required. Here we
describe a versatile and fully automated approach consisting of four
techniques, namely highly sensitive object detection, fuzzy logic based
dynamic object tracking, computer graphical visualization, and measurement
in time-space. Systematic model simulations were performed to evaluate the
reliability of the automated object detection and tracking method. To
demonstrate potential applications the method was applied to the analysis
of the functional dynamics of nuclear compartments enriched in pre-mRNA
splicing factors, of centromere motility during mitosis, and of rapid
secretory membrane traffic.
TIME-RESOLVED ANALYSIS AND VISUALIZATION OF DYNAMIC PROCESSES IN
LIVING CELLS
Tvarusk W 1, Bentele M 1, Misteli T 2, Rudolf R 3, Kaether C 3,
Gerdes HH 3, Spector DL 2, Eils R 1
1) Bioinformatics Group, Interdisciplinary Center of Scientific
Computing, University of Heidelberg, Germany,
2) Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, USA,
3) Department Neurobiology, University Heidelberg, Germany